Fig. 6.

Intact actin cytoskeletal reorganization is required for ROCK1-mediated glucose transport in 3T3-L1 adipocytes. A, Effects of latrunculin B on ROCK1-mediated glucose transport in 3T3-L1 adipocytes. Cells were transduced with recombinant adenovirus encoding β-Gal or ROCK1 as described in Materials and Methods. Cells were pretreated with latrunculin B (2 μm) or with vehicle for 3 h and then stimulated with insulin (3 or 100 nm) for 30 min. [³H]-2-deoxy-D-glucose uptake was measured, and the results are expressed as a percentage of basal glucose transport in control cells. Data are means ± sem and representative of three independent experiments. +, P < 0.001 vs. insulin-stimulated condition within same group; **, P < 0.001 vs. the corresponding condition of β-Gal. B, Effects of cytochalasin D on ROCK1-mediated glucose transport in 3T3-L1 adipocytes. Cells were transduced with recombinant adenovirus encoding β-Gal or ROCK1 as described in Materials and Methods. Cells were pretreated with cytochalasin D (2 μm) or with vehicle for 3 h and then stimulated with insulin (100 nm) for 30 min. [³H]-2-deoxy-D-glucose uptake was measured, and the results are expressed as a percentage of basal glucose transport in control cells. Data are means ± sem and representative of three independent experiments. +, P < 0.001 vs. insulin-stimulated condition within same group; *, P < 0.01; **, P < 0.001 vs. the corresponding condition of β-Gal.