Fig. 4.

Expression of the second ICL of CLR inhibits CGRP receptor signaling in NIH3T3 cells. A, CGRP-mediated cAMP response in NIH3T3 cells stably expressing ICL2^CLR, ICL3^CLR, or C-tail^CLR. B, Isoproterenol-mediated cAMP response in NIH3T3 cells stably expressing either ICL2^CLR or ICL2^β2AR. C, CGRP-mediated cAMP response in NIH3T3 cells expressing either ICL2^CLR or ICL2^β2AR. All data are presented as cAMP response normalized to maximal response obtained in untransfected NIH3T3 cells. All experiments were done in triplicate and repeated seven to eight times.