. 2012 Feb 7;153(4):1850–1860. doi: 10.1210/en.2011-1459

Table 2.

Effect of CLR ICL on cell-surface expression of CLR and β₂AR signaling

Receptor	ICL	Antibody	Surface expression (%)
HA-CLR + RAMP1	None	Anti-HA	100 ± 2
HA-CLR	None	Anti-HA	31 ± 6^b
HA-CLR + myc-RAMP1	ICL1^CLR	Anti-HA	90 ± 4
HA-CLR + myc-RAMP1	ICL2^CLR	Anti-HA	80 ± 5
HA-CLR + myc-RAMP1	ICL3^CLR	Anti-HA	108 ± 8
HA-CLR + myc-RAMP1	C-tail^CLR	Anti-HA	121 ± 7^a
HA-CLR + myc-RAMP1	ICL2^β2AR	Anti-HA	147 ± 2^b
HA-CLR + myc-RAMP1	None	Anti-myc	100 ± 4
myc-RAMP1	None	Anti-myc	15 ± 5^b
HA-CLR + RAMP1	ICL1^CLR	Anti-myc	76 ± 7
HA-CLR + myc-RAMP1	ICL2^CLR	Anti-myc	58 ± 9^b
HA-CLR + myc-RAMP1	ICL3^CLR	Anti-myc	59 ± 6^b
HA-CLR + myc-RAMP1	C-tail^CLR	Anti-myc	75 ± 9
HA-CLR + myc-RAMP1	ICL2^β2AR	Anti-myc	77 ± 6
HA-β2AR	None	Anti-HA	100 ± 3
HA-β2AR	ICL1^CLR	Anti-HA	77 ± 7
HA-β2AR	ICL2^CLR	Anti-HA	122 ± 19
HA-β2AR	ICL3^CLR	Anti-HA	118 ± 4
HA-β2AR	C-tail^CLR	Anti-HA	163 ± 26
HA-β2AR	ICL2^β2AR	Anti-HA	156 ± 36

Values are mean ± sem of four to five experiments carried out in triplicate. Surface expression was determined by ELISA for either HA (CLR and β₂AR) or myc (RAMP1) epitope in transfected COS1 cells. Significant differences from control (HA-CLR + RAMP1 alone or β₂AR alone) were detected by one-way ANOVA (followed by Tukey's test).

P < 0.05.

P < 0.01.