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. 2001 Apr 17;98(9):5294–5299. doi: 10.1073/pnas.091002298

Figure 3.

Figure 3

Inhibition of serine racemase by l-serine O-sulfate. (A) Enzyme activity was monitored at 37°C in media containing 50 mM Tris⋅HCl (pH 8.2), 10 mM l-serine, 10 μg/ml purified enzyme, 15 μM PLP, and different concentrations of l-serine O-sulfate (●). (B) Reaction medium and conditions were as described in A, except that l-serine concentration was varied from 1 to 20 mM either in the absence (○) or in the presence (●) of 8 mM l-serine O-sulfate. Inhibition exhibited a noncompetitive kinetics (Inset). The values are mean ± SEM of three different experiments with three enzyme preparations.