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pH-dependence of elimination and racemization reactions. (A) Elimination reaction was assayed for pyruvate formation at 37°C in media containing 50 mM Tris⋅HCl, 10 mM l-serine O-sulfate, 2 μg/ml recombinant enzyme, and 15 μM PLP (○). (B) Racemization of l-serine was assayed at 37°C in media containing 50 mM Tris⋅HCl, 10 mM l-serine, 10 μg/ml recombinant enzyme, and 15 μM PLP (●). The experiment was replicated three times, using different preparations with similar results.
