Table 1. Oligonucleotide primers for PCR and sequencing*.

Gene target	Primer sequence
PCR
16S rRNA (1,526 bp from ref. 20)
16S-FOR	5´ AGA GTT TGA TCC TGG 3´
16S-REV	5´ TAC CTT GTT ACG ACT T 3´
Tm = 55°C
16S rRNA signature sequence (298 bp from ref. 21)
16S1GF	5´ CGG CGT GGA TGA GGC AT 3´
16S1GR	5´ TCA GTC CCA GTG TTG GC 3´
Tm = 51°C
16S – 23S rRNA signature sequence (1 kbp from ref. 21)
16SF2	5´ CCG CCC GTC ACA TCA TGG 3´
23S1GR	5´ TGG CTC ATC ATG CAA AAG GCA 3´
Tm = 61°C
23S rRNA signature sequence (627 bp: domain I from ref. 21)
23S1GR	5´ TGG CTC ATC ATG CAA AAG GCA 3´
Tm = 61°C
MurA signature sequence (690 bp from ref. 22)
murA-for	5´ GTN GGN GCN ACN GAR AA 3'
murA-rev	5´ GCC ATN ACR TAN GCR AAN CCN GC 3´
Tm = 55°C
sctN (331 bp)
sctN FOR	5' AGA RGG AAT GAA ACG TTC 3'
sctN REV	5' GGC TCR TTC ATA TCA TC 3'
Tm = 58°C
Sequencing
16S rRNA (1,526 bp)
Forward:
F1 (M13)	5 GTT TTC CCA GTC ACG ACG TTG TA 3´
F2	5´ GCT CAC CAA GGC TAA GAC GTC 3´ (277-298)
F3	5´ CTA GCT TTG ACC TGA CGC TGA T 3´ (752-774)
F4	5´ GAA TCT GCA ACT CGG CTC CAT G 3´ (1323-1345)
Reverse:
R1 (M13)	5´ TTG TGA GCG GAT AAC AAT TTC 3´
R2	5´ CAT CCT AAA TGC TGG CAA C 3' (392-373)
R3	5´ CAC CGC TAC ATG TGG AAT TCC 3´ (843-822)
R4	5´ GAT CCT CTC TAG CAC CAT ATC C 3´ (1358-1336)

*PCR, polymerase chain reaction.