Table. Pneumocystis jirovecii genotyping by PCR-SSCP of 4 genomic regions*.
Patient | Date | SSCP pattern |
||||
---|---|---|---|---|---|---|
ITS1 | 26S | mt26 | β-tub | P. jirovecii type | ||
Index case-patient | 3/27/02 | A | A, B | A, B,C | A, B | >2 types (nonidentifiable) |
1 | 7/26/02 | A | A, B | A | A | 1, 2 |
2 | 10/30/02 | A | A, B | A | A | 1, 2 |
3 | 12/3/02 | A | A, B | A | A | 1, 2 |
4 | 2/7/03 | A, B | A, B, C | B, C | B, C | >2 types (nonidentifiable) |
5 | 2/15/03 | A | A, B | C | A | 6, 44 |
6 | 2/18/03 | A, B | A, B | A | C | 45, 46 |
*Bold letters signify the most abundant simple pattern with the complex one, as shown by silver staining. Bold numbers signify the most abundant P. jirovecii type. PCR, polymerase chain reaction; SSCP, multitarget single-strand conformation polymorphism.