Table 1. Primers used for PCR amplification and sequencing of Coxiella burnetii gene spacers.
| Spacer name | ORF | Nucleotide sequence (5´–3´)* | Amplified fragment length (bp) |
|---|---|---|---|
| Cox2 | Hypothetical protein | Cox20766 CAACCCTGAATACCCAAGGA | 397 |
| Hypothetical protein | Cox21004 GAAGCTTCTGATAGGCGGGA | ||
| Cox5 | Sulfatase domain protein | Cox77554 CAGGAGCAAGCTTGAATGCG | 395 |
| Entericidin, putative | Cox77808 TGGTATGACAACCCGTCATG | ||
| Cox18 | Ribonuclease H | Cox283060 CGCAGACGAATTAGCCAATC | 557 |
| DNA polymerase III, epsilon subunit | Cox283490 TTCGATGATCCGATGGCCTT | ||
| Cox20 | Hypothetical protein | Cox365301 GATATTTATCAGCGTCAAAGCAA | 631 |
| Hypothetical protein | Cox365803 TCTATTATTGCAATGCAAGTGG | ||
| Cox22 | Hypothetical protein | Cox378718 GGGAATAAGAGAGTTAGCTCA | 383 |
| Amino acid permease family protein | Cox378965 CGCAAATTTCGGCACAGACC | ||
| Cox37 | Hypothetical protein | Cox657471 GGCTTGTCTGGTGTAACTGT | 463 |
| Hypothetical protein | Cox657794 ATTCCGGGACCTTCGTTAAC | ||
| Cox51 | Replicative DNA helicase, intein-containing | Cox824598 TAACGCCCGAGAGCTCAGAA | 674 |
| Conserved hypothetical protein – Uridine kinase | Cox825124 GCGAGAACCGAATTGCTATC | ||
| Cox56 | OmpA-like transmembrane domain protein | Cox886418 CCAAGCTCTCTGTGCCCAAT | 479 |
| Conserved hypothetical protein | Cox886784 ATGCGCCAGAAACGCATAGG | ||
| Cox57 | Rhodanese-like domain protein | Cox892828 TGGAAATGGAAGGCGGATTC | 617 |
| Hypothetical protein | Cox893316 GGTTGGAAGGCGTAAGCCTTT | ||
| Cox 61 | Dioxygenase, putative | Cox956825 GAAGATAGAGCGGCAAGGAT | 611 |
| Hypothetical protein | Cox957249 GGGATTTCAACTTCCGATAGA |
*The numbers are beginning or end locations of the genes where the primers were chosen.