Table 1.
Antibodies
| Classification/Antibody | Clone | Dilution | Source |
|---|---|---|---|
| Cellular adhesion molecules | |||
| E-cadherin | NCH-38 | 1:100 | DakoCytomation, Carpinteria, CA, USA |
| CD44 | DF1485 | 1:400 | Novocastra, Newcastle upon Tyne, UK |
| Laminin5γ2 | 4G1 | 1:50 | DakoCytomation, Glostrup, Denmark |
| Growth factor | |||
| VEGF-C | Polyclonal | 1:50 | Abcam, Cambridge, UK |
| Apoptosis-associated proteins | |||
| bcl2 | 124 | 1:50 | DakoCytomation, Glostrup, Denmark |
| p53 | DO-7 | Pre-diluted | Nichirei, Tokyo, Japan |
| cleaved caspase-3 | Polyclonal | 1:400 | Cell signaling, Danvers, MA, USA |
| Mucin-related proteins | |||
| MUC1 | Ma695 | 1:100 | Novocastra, Newcastle upon Tyne, UK |
| MUC6 | CLH5 | 1:100 | Novocastra, Newcastle upon Tyne, UK |
| Hypoxia induced protein | |||
| HIF-1α | EP1215Y | 1:500 | Abcam, Cambridge, UK |
| Others | |||
| TTF-1 | 8G7G3/1 | 1:100 | DakoCytomation, Carpinteria, CA, USA |
| SP-A | PE10 | 1:100 | Dako, Kyoto, Japan |
| Vimentin | V9 | Pre-diluted | DakoCytomation, Carpinteria, CA, USA |
| Ki-67 | MIB-1 | 1:50 | Dako, Glostrup, Denmark |
| LYVE1 | 15A5B2 | 1:400 | Oriental Yeast, Tokyo, Japan |
| c-Met | EP1454Y | 1:200 | Abcam, Cambridge, UK |
| Phospho-c-Met | Polyclonal | 1:800 | Stressgen, Ann Arbor, MI, USA |
VEGF-C, vascular endothelial growth factor-C; HIF-1α, hypoxia induced factor 1-α; TTF-1, thyroid transcription factor-1; SP-A, surfactant apoprotein A; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1.