Figure 6. Viruses expressing Tip deficient in STAT6 activation fail to growth transform human T cells.

(A) Pairs of cell lines from identical donors are shown. Values of three different HVS transformed cell lines (2 donors #1587, 1611) are shown relative to the minimal transcript level detected for each cell line. (B). Human cord blood lymphocytes were infected with recombinant HVS strain C488 viruses, and maintained in medium supplemented with Interleukin-2. Cell growth was monitored and growth curves were calculated as described previously [28]. YYYY - recombinant wild type HVS in which Tip has four tyrosine residues Y94, Y114, Y127 and Y155; YFYY - mutant Y114F (tyrosine Y114 mutated to phenylalanine F); YYFY- mutant Y127F (Y127 mutated to F); YFFY- mutant Y114F and Y127F; FYYF - mutant Y94F and Y155F; FFFF - all four tyrosines mutated.