Figure 1. Functional data from genetic perturbation studies in autapses.

A) Confocal image of an autaptic neuron, scale bar is 50 µm. B) Schematic representation of a synapse. In each synapse only a limited number of vesicles, referred to as the readily releasable pool (RRP), is in a primed state that allows direct fusion during calcium triggering. The probability that a primed vesicle fuses during a calcium trigger is given by the vesicular release probability (P_v). C) Example traces of synaptic release variables. C1) Evoked EPSC (lower trace), upper trace shows somatic stimulation to trigger an action potential. C2) Postsynaptic response to a 500 mOsmol hypertonic stimulation for RRP estimation. C3) Spontaneous release events. D) Scatter plots of normalized and log-2 transformed synaptic variables in 2D projections of the 4D variable space for 121 perturbations (13 perturbations with >10-fold effect are not shown). *Non-genetic perturbations using a biochemical compound.