Figure 1. Demonstration of SsE-catalyzed conversion of PAF into lyso-PAF by TLC analysis.

PAF was incubated with 80 nM SsE or SsE^S178A for 10 min, and 1 µl of the reaction solution was analyzed by TLC, as described under Materials and Methods. Authentic PAF, lyso-PAF, and their mixture were included to verify their rates of migration.