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. 2012 Apr 5;8(4):e1002616. doi: 10.1371/journal.ppat.1002616

Figure 6. EGFR is phosphorylated upon flagella-mediated P. aeruginosa entry at the BL surface of polarized epithelium.

Figure 6

Calu-3 cells were grown as well polarized monolayers on Transwells for 9 days and treated with heparinase III (hepIII), tunicamycin (tun), or EGFR inhibitor (AG1478). As a control, cells were left untreated (un) or, as a positive control, cells were treated with HB-EGF. (A, B) PAO1, (C, D) PAO1ΔpilA, or (E, F) PAO1ΔfliC were added to the AP or BL chamber for 1 h. Lysates were immunoprecipitated with EGFR antibody followed by immunoblotting with phospho- or total-EGFR antibodies. Representative gels (A, C, E) and quantification by densitometry of three gels (B, D, F) are shown. The ratio of phospho-EGFR to total-EGFR for untreated cells was set to 1. Shown is the mean +/− SD for 3 independent experiments. **P<0.05 compared to cells BL infected with PAO1 (B) or PAO1ΔpilA (D).