Aliquots of purified T. brucei TfR (lanes 1, 2, 3, 6, 7, 10 and 11) and of bovine ribonuclease B, a positive control for ConA blotting (lanes 4 and 5), and bovine asialotransferrin, a positive control for ErCr lectin and ricin blotting (lanes 8, 9, 12 and 13), were separated by SDS-PAGE, transferred to nitrocellulose and subjected to blotting with anti-TfR antibody (lane 1), ConA (lanes 2–5), ErCr lectin (lanes 6–9) or ricin (lanes 10–13) in the absence (−) or presence (+) of the competing sugars α-methyl-mannose (lanes 3 and 5), lactose (lanes 7 and 9) or galactose and lactose (lanes 11 and 13). The positions of molecular weight markers are indicated for each group of blots.