Figure 3. Mixed-species biofilms formed with S. mutans ΔgtfBC::kan or with parental strain UA159 treated with mutanase.
The biofilms were formed in the presence of the gtfBC null mutant (A) or parental strain UA159 (B) with 1% (w/v) sucrose. Selected areas in (A) and (B) show detailed views of separated and merged confocal images of bacterial cells (green) and EPS (red). The biomass values of EPS and bacterial cells in the biofilms were calculated using COMSTAT. The data shown are mean values ± s.d. (n = 15).