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. 2012 Apr 5;7(4):e33920. doi: 10.1371/journal.pone.0033920

Figure 2. Ad5 vectors containing hexon hypervariable regions from Ad43 express PyCSP transgene efficiently.

Figure 2

Generation of Ad5 vectors with hypervariable regions of Ad43. A. Schematic showing the DE1 and FG1 loops of the unmodified Ad5 vector and three chimeric vectors containing Ad43 HVR substitutions in the FG1 loop, DE1 loop and both loops as indicated. B. Western blot showing the expression of the PyCSP antigen following infection of A549 cells with 200 pu/cell of purified vectors. Mock represents an uninfected cell lysate and serves as a negative control. ScPyCSP represents purified PyCSP protein from Saccharomyces cerevisiae and serves as a positive control. Hexon designations are indicated above the associated well (H = hexon) and the serotype source of the DE1 and FG1 loops shown in parentheses (DE1–FG1). Immunoblots were probed with antibody specific for PyCSP.