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. 2012 Apr 5;7(4):e33791. doi: 10.1371/journal.pone.0033791

Table 1. Expression of functionally related genes of R. sphaeroides 2.4.1ΔcryB compared to wild type under various conditions.

Ratio of expression level mutant/wild type
Category and RSP gene annotation Gene microaerobic non-stress conditions semiaerobic 60 min. blue light aerobic 20 min. 1O2 Description
Photosynthesis
RSP_0256 pufM 0.98 0.55 0.72 Photosynthesis reaction center M subunit
RSP_0257 pufL 1.06 0.49 0.99 Photosynthesis reaction center L subunit
RSP_0291 puhA 1.08 0.60 0.71 Reaction center H protein
RSP_0314 pucB 1.40 (0.52) 1.32 LHII light harvesting B800/850 protein
RSP_1518 prrA 1.04 0.61 0.65 PrrA (RegA) response regulator
RSP_1520 prrB (1.10) (0.37) (0.39) Sensor histidine kinase PrrB (RegB)
RSP_6108 pufB 0.68 0.68 0.84 LHI light harvesting B875 subunit
Citric acid cycle
RSP_4047 pdhAa 0.62 0.42 0.40 Pyruvate dehydrogenase E1 component
RSP_4049 pdhAb 0.58 0.36 0.35 Dihydrolipoamide acetyltransferase
RSP_4050 pdhB 0.55 0.39 0.39 Dihydrolipoamide acetyltransferase
Oxidative phosphorylation
RSP_0296 cycA 0.90 0.70 0.54 Cytochrome c2
RSP_0693 ccoP 1.12 0.59 0.61 Cbb3-type cytochrome c oxidase CcoP subunit
RSP_0694 ccoQ 1.09 0.56 0.63 Cbb3-type cytochrome c oxidase CcoQ subunit
RSP_0994 phaD (1.00) 0.39 0.61 NADH dehydrogenase subunit N
Stress response
RSP_0601 rpoHII (1.91) (0.61) 0.65 RNA polymerase sigma factor
RSP_1092 rpoE 1.21 0.62 0.58 RNA polymerase sigma-70 factor
RSP_1546 bfr 1.23 0.68 0.50 Bacterioferritin
RSP_2293 clpA (1.25) (0.65) 0.47 Chaperonin clpA/B
RSP_2346 0.57 0.35 0.43 Cold-shock DNA-binding domain protein
RSP_2389 (1.42) (0.64) 0.43 Putative glutathione peroxidase
RSP_2410 rpoHI 1.57 1.34 0.55 RNA polymerase sigma factor
Transcriptional regulators
RSP_0402 (1.19) (0.47) (0.61) Transcriptional regulator, TetR family
RSP_0847 1.73 2.01 0.57 Two component transcriptional regulator
RSP_0927 lyrS (1.09) (0.37) (0.78) Transcriptional regulator, LyrR family
RSP_3667 (0.70) 0.45 (0.64) Transcriptional regulator, AraC family
RNA processing/degradation
RSP_0224 (1.31) (0.49) (0.78) ATP-dependent helicase
RSP_1112 pnp 0.70 0.62 0.67 Polyribonucleotide nucleotidyltransferase
RSP_1126 rnr (1.17) (0.46) (0.61) Exoribonuclease R
RSP_1971 rnd (0.94) 0.40 (0.60) Ribonuclease D
RSP_2131 rne 1.07 0.60 0.71 Ribonuclease E
RSP_2843 hfq 1.20 0.59 0.57 RNA-binding protein Hfq
Others
RSP_0030 1.05 0.39 0.62 PAS sensor GGDEF/EAL domain
RSP_0905 sitB 1.20 0.75 2.10 ABC Mn+2/Fe+2 transporter, ATPase subunit
RSP_2877 coxL 1.11 0.71 0.29 Putative carbon monoxide dehydrogenase
RSP_3571 znuA 0.72 0.78 2.97 ABC zinc transporter
RSP_3539 0.47 0.35 0.43 Hemolysin-type calcium-binding region, RTX
RSP_3871 modA 3.48 (0.82) (1.10) ABC molybdate transporter
RSP_4157 2.91 (0.67) (0.53) Radical SAM superfamily protein
RSP_4158 2.30 (0.67) (0.63) Generic methyltransferase

Significant changes of the microarray data are in bold. Numbers in brackets failed to reach the set A-value criteria.