Table 1. Expression of functionally related genes of R. sphaeroides 2.4.1ΔcryB compared to wild type under various conditions.
Ratio of expression level mutant/wild type | |||||
Category and RSP gene annotation | Gene | microaerobic non-stress conditions | semiaerobic 60 min. blue light | aerobic 20 min. 1O2 | Description |
Photosynthesis | |||||
RSP_0256 | pufM | 0.98 | 0.55 | 0.72 | Photosynthesis reaction center M subunit |
RSP_0257 | pufL | 1.06 | 0.49 | 0.99 | Photosynthesis reaction center L subunit |
RSP_0291 | puhA | 1.08 | 0.60 | 0.71 | Reaction center H protein |
RSP_0314 | pucB | 1.40 | (0.52) | 1.32 | LHII light harvesting B800/850 protein |
RSP_1518 | prrA | 1.04 | 0.61 | 0.65 | PrrA (RegA) response regulator |
RSP_1520 | prrB | (1.10) | (0.37) | (0.39) | Sensor histidine kinase PrrB (RegB) |
RSP_6108 | pufB | 0.68 | 0.68 | 0.84 | LHI light harvesting B875 subunit |
Citric acid cycle | |||||
RSP_4047 | pdhAa | 0.62 | 0.42 | 0.40 | Pyruvate dehydrogenase E1 component |
RSP_4049 | pdhAb | 0.58 | 0.36 | 0.35 | Dihydrolipoamide acetyltransferase |
RSP_4050 | pdhB | 0.55 | 0.39 | 0.39 | Dihydrolipoamide acetyltransferase |
Oxidative phosphorylation | |||||
RSP_0296 | cycA | 0.90 | 0.70 | 0.54 | Cytochrome c2 |
RSP_0693 | ccoP | 1.12 | 0.59 | 0.61 | Cbb3-type cytochrome c oxidase CcoP subunit |
RSP_0694 | ccoQ | 1.09 | 0.56 | 0.63 | Cbb3-type cytochrome c oxidase CcoQ subunit |
RSP_0994 | phaD | (1.00) | 0.39 | 0.61 | NADH dehydrogenase subunit N |
Stress response | |||||
RSP_0601 | rpoHII | (1.91) | (0.61) | 0.65 | RNA polymerase sigma factor |
RSP_1092 | rpoE | 1.21 | 0.62 | 0.58 | RNA polymerase sigma-70 factor |
RSP_1546 | bfr | 1.23 | 0.68 | 0.50 | Bacterioferritin |
RSP_2293 | clpA | (1.25) | (0.65) | 0.47 | Chaperonin clpA/B |
RSP_2346 | 0.57 | 0.35 | 0.43 | Cold-shock DNA-binding domain protein | |
RSP_2389 | (1.42) | (0.64) | 0.43 | Putative glutathione peroxidase | |
RSP_2410 | rpoHI | 1.57 | 1.34 | 0.55 | RNA polymerase sigma factor |
Transcriptional regulators | |||||
RSP_0402 | (1.19) | (0.47) | (0.61) | Transcriptional regulator, TetR family | |
RSP_0847 | 1.73 | 2.01 | 0.57 | Two component transcriptional regulator | |
RSP_0927 | lyrS | (1.09) | (0.37) | (0.78) | Transcriptional regulator, LyrR family |
RSP_3667 | (0.70) | 0.45 | (0.64) | Transcriptional regulator, AraC family | |
RNA processing/degradation | |||||
RSP_0224 | (1.31) | (0.49) | (0.78) | ATP-dependent helicase | |
RSP_1112 | pnp | 0.70 | 0.62 | 0.67 | Polyribonucleotide nucleotidyltransferase |
RSP_1126 | rnr | (1.17) | (0.46) | (0.61) | Exoribonuclease R |
RSP_1971 | rnd | (0.94) | 0.40 | (0.60) | Ribonuclease D |
RSP_2131 | rne | 1.07 | 0.60 | 0.71 | Ribonuclease E |
RSP_2843 | hfq | 1.20 | 0.59 | 0.57 | RNA-binding protein Hfq |
Others | |||||
RSP_0030 | 1.05 | 0.39 | 0.62 | PAS sensor GGDEF/EAL domain | |
RSP_0905 | sitB | 1.20 | 0.75 | 2.10 | ABC Mn+2/Fe+2 transporter, ATPase subunit |
RSP_2877 | coxL | 1.11 | 0.71 | 0.29 | Putative carbon monoxide dehydrogenase |
RSP_3571 | znuA | 0.72 | 0.78 | 2.97 | ABC zinc transporter |
RSP_3539 | 0.47 | 0.35 | 0.43 | Hemolysin-type calcium-binding region, RTX | |
RSP_3871 | modA | 3.48 | (0.82) | (1.10) | ABC molybdate transporter |
RSP_4157 | 2.91 | (0.67) | (0.53) | Radical SAM superfamily protein | |
RSP_4158 | 2.30 | (0.67) | (0.63) | Generic methyltransferase |
Significant changes of the microarray data are in bold. Numbers in brackets failed to reach the set A-value criteria.