Figure 5. PscRXLR1 is a splice variant of Psc_781.4.

(A) Schematic representation of intron and exon structures of PITG_17484, Psc_781.4, and PscRXLR1. (B) RT-PCR analysis of alternative splicing in Psc_781.4 in sporangia (SP) and at 4 and 8 days post-inoculation (dpi). RT-PCR products were subcloned into the pGEM vector and DNA was amplified by PCR using intron spanning primers as shown in (A). S, transcript with spliced intron 1. NS, non-spliced transcript.