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. 2012 Apr 5;7(4):e34495. doi: 10.1371/journal.pone.0034495

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Azevedo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Neighbor-joining tree of putative astacin from L. longipalpis (RAAPBAR022F08 male reproductive organs cDNA libraries), L. longipalpis 2 (AM088883 whole female cDNA libraries) and L. longipalpis 3 (Lulo-Astacin A8CW49_LUTLO, midgut female library) A. aegypti (AAEL013449), A. gambiae (AGAP010764), D. melanogaster (FBpp0080341/CG15254) and Nasonia vitripenis (NV12552). Bootstrap percentage values indicated in nodes are based on 1000 replicates. (B) Multiple alignment of putative astacin of male reproductive tracts from L. longipalpis and its orthologues in Diptera. Conserved amino acids are indicated by (*).