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. 2012 Apr 5;7(4):e34495. doi: 10.1371/journal.pone.0034495

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Azevedo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Neighbor-joining tree of putative protease inhibitor L. longipalpis (RAAPBAR023H02/EW989852 B male reproductive organs and midgut female cDNA library), A. aegypti (AAEL000551), A. gambiae (AGAP011319), and Apis mellifera (XP_003250953). Bootstrap percentage values indicated in nodes are based on 1000 replicates. (B) Multiple alignment of putative protease inhibitor of male reproductive tracts from L. longipalpis and its orthologues in Diptera. Conserved amino acids are indicated by (*).