Skip to main content
. 2012 Apr 5;7(4):e35244. doi: 10.1371/journal.pone.0035244

Figure 1. MN marker gene expression in hMSCs expressing Olig2 and Hb9 in the presence of MNIM.

Figure 1

(A) Schematic illustration of the protocol used for MN induction of hMSCs. (B) RT-PCR analyses of hMSCs expressing EGFP (E), EGFP-Olig2 (EO), EGFP-Hb9 (EH), and EGFP-Olig2-Hb9 (EOH) with MNIM treatment were preformed. Quantification of NF-M (C), Islet-1 (D), and VAChT (E) PCR products. The mRNA level of a given gene was quantified by densitometry and normalized to the corresponding β-actin level. The bars represent mean±SEM of at least three independent experiments. *P<0.05 versus uninduced E. The significance was determined by Mann-Whitney test with Fisher’s LSD post hoc test.