Figure 2. Doxycycline-inducible knockdown of BRG1 and BRM in MCF-10A cells.
Total protein was extracted from cells treated or not for 3 days with 0.01 µg/ml doxycycline. BRG1 (A), BRM (B) and GFP protein expression were examined by Western blotting. The protein of 0.5 × 105 cells per lane was separated by SDS-PAGE (7.5 %) and probed with the indicated antibodies. PI3 kinase was the loading control.