(A) Growth curves for MCF-10A cells after BRG1 or BRM shRNA knockdown, or expressing a scrambled sequence shRNA (SCRAM). Cells were pre-incubated for 2 days with 0.05 µg/ml doxycycline (marked +) before being seeded in a 12-well dish at 1500 cells per well at day 0. Control cells (marked −) were not treated with doxycycline and did not express the shRNA. Cells were trypsinized and then counted each day from day 2 to day 5.
(B) Cell proliferation was measured every day by quantifying DNA(Cyquant kit) in parallel with the cell counting of panel A. The decrease of proliferation was quantified by calculating the ratio (in percent) of the cell number with doxycycline to the cell number of the matched doxycycline-free control.