(A) Effects of combinations of 0.1 mM SA, 0.5 mM APAF,
and/or 1.0 mM caffeine for 4 days on rapidly proliferating
mIMCD3. The cell number was measured by MTT after 4 days of exposure to
the drugs. (B) Effects of SA, APAF, and caffeine on
confluent mIMCD3 cells. The concentration of each drug was 2.0 mM.
After 4 days of exposure to the drugs, the cell number was measured by
MTT (mean ± SEM, n = 4, *,
P < 0.05 versus APAF alone). FBS was present
during the experimental period in two experiments, but not in the other
two. Because the results do not differ, they are combined.