The Anti-DDR1 mAbs Bind to the DS-Like Domain and Do Not Inhibit Ligand Binding
(A) ELISA showing binding of the indicated anti-DDR1 mAbs to recombinant DDR proteins immobilized on 96-well plates. Shown is a representative of three independent experiments, each performed in duplicate.
(B) Solid-phase binding assay with recombinant DDR1-Fc protein added to 96-well plates coated with either casein or collagen peptide III-23 (Xu et al., 2011). DDR1-Fc was preincubated with the indicated anti-DDR1 mAbs before addition to the wells. Bound DDR1-Fc was detected with anti-human Fc Ab and was measured as absorbance at 492 nm. Shown is a representative of three independent experiments, each performed in triplicate. The error bars indicate the sample standard deviation (n = 3).