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. 2012 Apr 4;20-178(4):688–697. doi: 10.1016/j.str.2012.02.011

Figure 2.

Figure 2

The Anti-DDR1 mAbs Bind to the DS-Like Domain and Do Not Inhibit Ligand Binding

(A) ELISA showing binding of the indicated anti-DDR1 mAbs to recombinant DDR proteins immobilized on 96-well plates. Shown is a representative of three independent experiments, each performed in duplicate.

(B) Solid-phase binding assay with recombinant DDR1-Fc protein added to 96-well plates coated with either casein or collagen peptide III-23 (Xu et al., 2011). DDR1-Fc was preincubated with the indicated anti-DDR1 mAbs before addition to the wells. Bound DDR1-Fc was detected with anti-human Fc Ab and was measured as absorbance at 492 nm. Shown is a representative of three independent experiments, each performed in triplicate. The error bars indicate the sample standard deviation (n = 3).