Table 1.
Crystallographic Statistics of the DDR1-3E3 Fab Structure
| Data Collection | Value |
|---|---|
| Space group | C2221 |
| Unit cell dimensions | |
| a, b, c (Å) | 102.51, 251.48, 75.37 |
| α, β, γ (°) | 90, 90, 90 |
| Asymmetric unit content | 1:1 DDR1-3E3 Fab complex |
| Solvent content (%) | 57 |
| Resolution (Å) | 50–2.8 (2.95–2.80)a |
| Rmerge | 0.079 (0.429) |
| <I/σ(I) > | 14.3 (3.8) |
| Completeness (%) | 98.7 (98.9) |
| Multiplicity | 6.0 (6.1) |
| Refinement | |
| Resolution (Å) | 20–2.8 |
| Reflections | 24035 |
| Protein atoms | 2700 (DDR1) + 3169 (3E3 Fab) |
| Solvent atoms | 2 Ca2+ + 14 H2O |
| Rwork/Rfree | 0.216/0.286 |
| Rmsd bonds (Å) | 0.007 |
| Rmsd angles (°) | 1.4 |
| Average B-factor (Å2) | 53.6 |
| Ramachandran plot (%)b | 91.3/98.1 |
a
Values in parentheses are for the highest resolution shell.
b
Percentage of residues in favored and allowed regions (Chen et al., 2010).