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. Author manuscript; available in PMC: 2012 Apr 6.
Published in final edited form as: Science. 2011 May 13;332(6031):852–855. doi: 10.1126/science.1202143

Figure 1. notum is expressed at anterior-facing wounds.

Figure 1

(A-C) notum in situ hybridizations: intact animals (A); regenerating head and trunk fragments over time (hours, h) (B, C). (A) Brackets: regions imaged in (B, C). (D) notum is expressed in anterior-pole, subepidermal cells (arrow). (D, E) Dotted green line is enlarged in right panels. (E) Double-fluorescence in situ hybridization (FISH); notum (red) and wnt1 (blue) are co-expressed (arrows) at an anterior-facing wound (dotted line) 18h after amputation. (F-I) FISH; notum expression 6h after incisions. Above, green box depicts region imaged. Red lines depict incisions. Below, red line shows sealed wound location. In (G, H) triangular tissue was removed and the wound allowed to seal; tissue between incision sites (~200 microns apart) in (I) was not removed. Anterior, left (B-I) or top (A). Dorsal view (A, 72h in C, D), or ventral view (all others). Images represent ≥4 of 5 animals per panel. Bars, 200 microns.

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