Fig. 1.
Database-screened antimicrobial peptide 1 (DASamP1) impacts Staphylococcus aureus biofilms in vivo. C57BL/6 mice (Day 3, n = 16/group; Day 14, n = 8/group) were infected with 103 colony-forming units (CFU) of MRSA USA300 LAC::lux in the lumen of surgically implanted catheters to establish biofilm infection. Animals received DASamP1 injections at 0, 24 and 48 h following infection and were sacrificed at Day 3 (A,B) or Day 14 (C,D) following S. aureus exposure, whereupon catheters (A,C) and surrounding host tissues (B,D) were recovered to quantitate bacterial burdens. Results are expressed as the number of CFU/mL for catheters or CFU/mg tissue in order to correct for differences in tissue sampling size. * Significant difference in bacterial burden between phosphate-buffered saline (PBS)- and peptide-treated mice (P < 0.05). Results presented from individual animals in two independent experiments, with bars representing the mean of each group.