Table 2.
Antimicrobial and haemolytic activities of the six anti-meticillin-resistant Staphylococcus aureus peptides identified in this study
| Peptide | MIC (μM)
|
HL50 (μM)a | CSb | Net chargec | Pho%c | RT (min)d | |
|---|---|---|---|---|---|---|---|
| Bacillus subtilis | Pseudomonas aeruginosa | ||||||
| Ascaphin-8 | 6.25 | 12.5 | 45 | 15 | +4 | 55 | 10.08 |
| DASamP1 | >100 | >100 | 25 | 8 | +5 | 61 | 12.83 |
| DASamP2 | 12.5 | 6.25 | 75 | 12 | +7 | 57 | 10.73 |
| Lycotoxin I | 6.25 | 25 | 125 | 40 | +7 | 50 | 10.94 |
| Maculatin 1.3 | 25 | >100 | 25 | 4 | +3 | 54 | 15.15 |
| Piscidin 1 | 12.5 | 50 | 20 | 7 | +7 | 43 | 14.68 |
MIC, minimal inhibitory concentration.
a
Concentration at which 50% of red blood cells were lysed.
b
Cell selectivity (CS) of the peptide against S. aureus USA300 LAC was calculated as HL50/MIC (MICs in Table 1).
c
Net charge and hydrophobic residues% (pho%) were calculated using the Antimicrobial Peptide Database [7].
d
High-performance liquid chromatography retention time (RT) measured as described previously [10].