Figure 4. Allele specific distribution of chromosomal translocations in F1 A-MuLV Transformants.

A, B) V(D)J recombination substrates ("DEL-CJ") were integrated into 129/BALB/cJ pro-B genome within chr7 BALB/cJ allele in one line (A; "Chr7^DEL-CJ") and the chr9 129 allele in a different line (B; "Chr9^DEL-CJ"). Translocation libraries from the V(D)J substrates were generated by HTGTS and translocations were mapped to the BALB/cJ versus 129 alleles based on available SNPs. The circos plots show the distribution of allelic specific junctions within the two copies of chr7 (A) and the two copies of chr9 (B) for the Chr7^DEL-CJ and Chr9^DEL-CJ lines, respectively. Individual translocations are represented as arcs originating from I-SceI DSBs and terminating at the partner site in BALB/cJ (left) or 129 (right) chromosomes. The 2 Mb chromosomal region spanning substrate integration sites was omitted from analyses. C) Bar graphs show the relative allelic distribution of translocations on the BALB/cJ and 129 derived chromosomes 7 and 9 for V(D)J recombination substrates integrated into chr7 and chr9. The 2 Mb chromosomal region spanning the substrate integration site was omitted from analyses. See also Fig. S4.