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. 2012 Apr 6;7(4):e35265. doi: 10.1371/journal.pone.0035265

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Ma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Liver (L) and pituitary gland (P) were used as the negative and positive controls respectively. Mouse 18s rRNA was chosen as an internal control. (B) A breast cancer (BC) cell line was used as the positive control. (C) n = 3 for Western blots, data are shown as means ± S.E.M. and analyzed by One-way ANOVA with Tukey’s post hoc test. ***P < 0.001 vs BC group. GHS-R1a mRNA and protein are expressed in the left ventricle (LV), septum (S) and right ventricle (RV) of the mouse heart.