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. 2012 Apr 6;7(4):e35074. doi: 10.1371/journal.pone.0035074

Figure 1. Optimization of relative fitness calculations for chimeric virus competitions.

Figure 1

Infection of untreated Jurkat cells with BaL- and HxB2-Env-supplemented pBRNL43 IRES eGFP/DsRedExpress constructs; fluorescence levels were assessed by flow cytometry after 7 days of infection. A) Live gate; B) regions of eGFP+, DsRedExpress+ and double+; C) eGFP+ cells in BaL mono-infected cultures; D) DsRedEpress+ cells in HxB2 mono-infected cultures; E) eGFP+, DsRedExpress+ and double+ cells in BaL/HxB2 double-infected cultures.