Figure 3. Relative fitness of R5 versus X4 HIV strains in presence and absence of visfatin and maraviroc.

Flow cytometric evaluation of infection rates of Jurkat T cells by eGFP and DsRedExpress chimeric viruses at 0.1 MOI in presence and absence of visfatin (200 ng/ml) or maraviroc (5 nM): A) HIV_943-3 (R5) versus HIV_943-1 (X4) and B) HIV_BaL (R5) versus HIV_HxB2 (X4). Ratios are depicted of infection rates in double infections normalised to the mono-infection rates; all infections were performed in a colour-flipped setup. Mean and SEM were calculated based on the colour-flipped datasets within each experiment; representative results for three independent experiments are shown. NT: untreated control.