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. 2012 Apr 6;7(4):e28706. doi: 10.1371/journal.pone.0028706

Figure 3. Effect of Icariside II on apoptosis-related proteins in U937 cells.

Figure 3

(A) U937 cells were treated with Icariside II (50 µM) for 0, 6, 12 or 24 h. Cell lysates were prepared and subjected to Western blotting for procaspase-3 and PARP. (B) HL-60 cells were treated with or without Icariside II (50 µM) for 24 h. Western blot analysis was performed for PARP. (C) U937 cells were treated with Icariside II (50 µM) for 0, 6, 12 or 24 h. Western blotting was performed for bcl-2, bcl-xL, survivin and COX-2. Representative results of three independent experiments are shown for each experiment.