Figure 7. Effects of Icariside II on STAT3-related signaling in STAT3 inactive MM.1S cells.

(A) MM.1S cells were treated with or without 50 µM Icariside II for 9 h. Cell lysates were prepared and subjected to Western blotting for phospho-STAT3 and phospho-STAT5. U937 cells were used as a control of phospho-STAT3 and 5. (B) MM.1S cells were treated with or without 50 µM Icariside II for 9 h. Cell lysates were prepared and subjected to Western blotting for phospho-JAK2, phospho-Src and SHP-1. (C) MM.1S cells were treated with or without 50 µM Icariside II for 24 h. Cell lysates were prepared and subjected to Western blotting for procaspase-3, PARP, bcl-x_L, bcl-2, survivin and COX-2. Representative results of three independent experiments are shown for each experiment.