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. 2012 Apr 6;7(4):e33235. doi: 10.1371/journal.pone.0033235

Figure 1. Measurement of rHBsAg VLP antigenicity by SPR or solution competitive ELISA.

Figure 1

(A) Solution competition ELISA measurement (or relative IC50s) for freshly prepared (n = 5), heat-treated (n = 5), and redox-refolded (n = 3) rHBsAg VLPs; (B) A1.2 epitope maturation over time in PBS and with a redox buffer-reduced (GSH) and/or oxidized (GSSG) forms of glutathione; (C) relative antigenicity by SPR with A1.2 and RF1 (n = 12); (D) Relative antigenicity of rHBsAg in aqueous solution as well as when adsorbed onto particulate adjuvant.