Figure 3. Ectopic overexpression of miR-22 in AR42J-B13 cells was analyzed by Northern blot (A) and stem-loop real-time PCR analysis (B).

(A) pIRES is the empty vector control. pIRES-miR-22 is the miR-22 expression vector. (B) B13-pIRES is the AR42J-B13 cells stably transfected with the pIRES vector control, while B13-pIRES-miR-22 is the AR42J-B13 cells stably transfected with the miR-22 expression vector. MicroRNA-93 was used as a negative control. (***, p<0.001).