Figure 4. 2D-DIGE analysis identified differentially expressed proteins in miR-22 overexpressing AR42J-B13 cells.

Protein lysates of pooled B13-pIRES or B13-pIRES-miR-22 cells were labeled with Cy3 and Cy5, respectively (Experimental Procedures). Three differentially expressed features, Features A, B and C, were picked up for identification by LC-MS/MS. Proteins with altered expression were indicated by arrows. The candidate proteins identified were listed in the table below the image. Parathymosin protein was down-regulated as assayed by 2D-DIGE (A) and Western blot analysis (C) in AR42J-B13 cells stably transfected with pIRES-miR-22. The miR-22 expression level in transiently transfected AR42J-B13 cells was measured by stem-loop real-time PCR (B). (***, p<0.001).