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. 2012 Apr 6;7(4):e34116. doi: 10.1371/journal.pone.0034116

Figure 5. Reduction of parathymosin mRNA and protein levels were observed in AR42J-B13 cells treated with Dex/OSM or a miR-22 expression vector.

Figure 5

(A) Reduced expression of parathymosin protein in AR42J-B13 cells was induced by Dex/OSM treatment (B13+DM) or by stable expression of miR-22 using Western blot analysis. (B & C) The expression of parathymosin mRNA was reduced to approximately 70% level in Dex/OSM induced AR42J-B13 cells (B), and in the stably transfected AR42J-B13 cells overexpressing miR-22 (C). The mRNA level was measured by real-time PCR analysis (***, p<0.001).