Figure 7. Compensatory mutations of miR-22 restored the repression effect of miR-22 on the 3′UTR of a parathymosin reporter containing a mu2 target site.

(A) Compensatory mutation from 5′-CC-3′ to 5′-GG.-3′ at the predicted seed sequences of miR-22. (B) Reporter gene assay was performed by co-transfection into AR42J-B13 cells with the various DsRED reporter plasmids, containing various wild type and mutant parathymosin 3′UTR, and pIRES-miR-22 containing the compensatory mutation. FACS analysis was performed as in Fig. 6. (***, p<0.001; ns, not significant).