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. 2012 Apr 6;7(4):e34116. doi: 10.1371/journal.pone.0034116

Figure 10. The potential effect of parathymosin or miR22 on the transdifferentiation of AR42J-B13 cells.

Figure 10

(A) Similar levels of HBV e antigen (HBeAg) were detected in the media of B13-1 cells transfected with LNA anti-miR22 vs. a LNA scramble control. (B) Increased secretion of HBV surface antigen (HBsAg) was detected by ELISA in the medium of B13-1 cells transfected with LNA anti-miR22. (C) The reduction of the endogenous level of miR-22 in B13-1 cells after LNA anti-miR-22 treatment, was measured by real time PCR. (D) Treatment of B13-1 cells with LNA anti-miR22 resulted in no significant effect on transdifferentiation markers of alpha1-antitrypsin and albumin by Western blot analysis. (E & F) No significant effect on secreted HBsAg and HBeAg was detected by ELISA in B13-1 cells transfected with a parathymosin (PTMS) expression vector. (G) A time course of the gradual decrease of parathymosin protein in B13-1 cells was conducted by Western blot analysis. B13-1 cells were transfected with a vector control (upper panel) or a parathymosin expression vector (lower panel) prior to Dex/OSM induction. (H) Transdifferentiation markers of albumin and alpha1-antitrypsin were measured by Western blot analysis, using B13-1 cells transfected with a parathymosin expression vector or a control vector pCDNA prior to Dex/OSM induction.