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Antibody pull-downs of IFT A reveal formation of a subcomplex in ift121. Aliquots of soluble protein from whole cell extracts of CC-503 or ift121 were incubated with preimmune (PI) or subunit-specific antibody resins as indicated above each column. Immunoadsorbed proteins were resolved by SDS-PAGE and transferred to nitrocellulose and probed with anti-IFT A antibodies indicated to the left of each row.
