FIGURE 3.

Protein oxidation in chloroplasts isolated from tAPX-silenced leaves. Seventeen-day-old IS-GUS-2-17 and IS-tAPX-19-23 plants were sprayed with a 100 μm estrogen and kept under NL. At 48 h after estrogen treatment, the chloroplasts were isolated from leaves of IS-GUS-2-17 and IS-tAPX-19-23 plants. A, oxidized proteins in the crude extracts and chloroplasts were detected by Western blotting. The same results were obtained in five independent experiments. Two representative results are shown as Exp. 1 and 2. CBB, Coomassie Brilliant Blue. B, shown are Western blots of tAPX, sAPX, and APX1 for Exp. 1. The blot of APX1 indicated that there was no contamination of the cytosolic fraction in the chloroplasts. In the figure, IS-GUS-2-17 and IS-tAPX-19-23 plants are shown as IS-GUS and IS-tAPX, respectively.