FIGURE 4.

Effects of treatment with AsA or dark on the induction of RTS genes by tAPX-silencing. A, seventeen-day-old IS-GUS-2-17 and IS-tAPX-19-23 plants were sprayed with a 100 μm estrogen and kept under NL or dark. At 45 h after the treatment with estrogen, the leaves of IS-GUS-2-17 and IS-tAPX-19-23 plants were sprayed with 10 mm AsA or water and kept under NL. The transcript levels of five RTS genes (GRFP, JLFP, ULT2, CYP72A14, and PK) were measured by q-PCR as described under “Experimental Procedures.” Error bars indicate S.D. (n = 3). Values without a common letter are significantly different (p < 0.05). B, transcript levels of five RTS genes (described in Fig. 4A) in the wild-type and KO-tAPX plants, grown under light for 17 days, were measured by q-PCR. Error bars indicate S.D. (n = 3). Significant differences: *, p < 0.05 versus the value for wild-type plants.