FIGURE 9.
Effect of tAPX silencing on the expression of RTS genes under ML. One-week-old IS-GUS-2-17 and IS-tAPX-19-23 plants grown under NL were further grown for 10 days under ML (400 μmol of photons m−2 s−1). The plants were then sprayed with estrogen and kept under ML for 72 h. A, 48 h after estrogen treatment the IS-GUS-2-17 and IS-tAPX-19-23 plants were photographed. B, Fv/Fm values in the leaves of IS-GUS-2-17 and IS-tAPX-19-23 after estrogen treatment were measured using a Closed FluorCam 800MF. Error bars indicate S.D. (n = 3). C, 48 h after estrogen treatment, the transcript levels of RTS genes (GRFP, JLFP, ULT2, CYP72A14, and PK) were measured by q-PCR. Error bars indicate S.D. (n = 3). Error bars indicate S.D. (n = 3). Significant differences: *, p < 0.05 versus the value for IS-GUS-2-17 plants.