FIGURE 2.

Validation of the dual color FRAP approach. a–d, quantitative dual color FRAP analysis of stable transfectants co-expressing WT H1⁰-ChFP and WT H1⁰-GFP (a), H1⁰(K73A)-ChFP and H1⁰(K73A)-GFP (b), WT H1⁰-ChFP and H1⁰(K73A)-GFP (c), and H1⁰(K73A)-ChFP and WT H1⁰-GFP (d). The values for the half-time of recovery (t₅₀) were determined as previously described (35) and represent the means ± S.D. of at least eight independent measurements from a pool of three stable cell lines. The error bars are omitted from the plots for clarity. Table 1 provides the corresponding statistical analyses.