FIGURE 4.

Identification of conserved residues in the globular domains of H1⁰ and H1c showing differential binding behavior. a and b, plots showing recovery kinetics of H1⁰ mutants H1⁰(K52A)-GFP (a) and H1⁰(K85A)-GFP (b), relative to co-expressed H1⁰-ChFP. c and d, plots showing recovery kinetics of H1c mutants H1c(K63A)-GFP (c) and H1c(K96A)-GFP (d), relative to co-expressed H1c-ChFP. The values for the half-time of recovery (t₅₀) were determined as previously described (35) and represent the means ± S.D. of at least eight independent measurements from a pool of three stable cell lines. The error bars are omitted from the plots for clarity. Table 2 provides the corresponding statistical analyses.