FIGURE 6.

Quantitative FRAP analysis on cells expressing N-terminal domain swap bearing mutations in the globular domain. a–f, dual color FRAP analysis with cells co-expressing 0CC-GFP and 0CC-ChFP (a), 0C(K63A)C-GFP and 0CC-ChFP (b), 0C(K96A)C-GFP and 0CC-ChFP (c), C00-GFP and C00-ChFP (d), C0(K52A)0-GFP and C00-ChFP (e), and C0(K85A)0-GFP and C00-ChFP (f). The values for the half-time of recovery (t₅₀) were determined as previously described (35) and represent the means ± S.D. of at least eight independent measurements from a pool of three stable cell lines. The error bars are omitted from the plots for clarity. Table 2 provides the corresponding statistical analyses.