TABLE 1.
Quantitative analyses validating the dual color FRAP approach
| Construct |
t50a |
t50 ratio of GFP/ChFP | p valueb | |
|---|---|---|---|---|
| ChFP | GFP | |||
| s | ||||
| 0(WT)ChFP/0(WT)GFP | 28.1 ± 7.2 (0.26) | 28.4 ± 6.5 (0.23) | 0.99 ± 0.08 (0.08) | 0.4115 |
| 0(K73A)ChFP/0(K73A)GFP | 15.7 ± 2.3 (0.15) | 16.2 ± 2.5 (0.15) | 0.97 ± 0.06 (0.06) | 0.2758 |
| 0(WT)ChFP/0(K73A)GFP | 28.7 ± 4.6 (0.16) | 15.6 ± 2.8 (0.15) | 0.54 ± 0.02 (0.04) | 0.0001 |
| 0(K73A)ChFP/0(WT)GFP | 15.8 ± 3.8 (0.26) | 30.7 ± 6.3 (0.2) | 0.52 ± 0.02 (0.04)c | 0.0001 |
a The values for t50 were determined as previously described (35). The values are the means ± S.D. from at least eight independent measurements. The values in parentheses denote the calculated coefficients of variation.
b Paired Student's t test for H1-GFP versus H1-ChFP.
c The ratio of GFP/ChFP was inverted to ChFP/GFP for comparison.