TABLE 2.
Quantitative analysis of H10 versus H1c and domain swap mutants
| Construct |
t50a |
t50 ratio of GFP/ChFP | p valueb | |
|---|---|---|---|---|
| ChFP | GFP | |||
| s | ||||
| 0-ChFP/C-GFP | 30.3 ± 1.9 (0.06) | 22.7 ± 1.6 (0.07) | 0.75 ± 0.03 (0.03) | 0.0001 |
| C-ChFP/0-GFP | 23.1 ± 4.6 (0.2) | 30.5 ± 7.0 (0.23) | 0.77 ± 0.05 (0.07) | 0.0002 |
| 0-ChFP/00C-GFP | 28.7 ± 3.8 (0.13) | 29.2 ± 4.2 (0.14) | 1.02 ± 0.06 (0.06) | 0.4066 |
| 0-ChFP/CC0-GFP | 30.1 ± 3.9 (0.13) | 23.3 ± 3.8 (0.16) | 0.77 ± 0.04 (0.06) | 0.0001 |
| 0-ChFP/0CC-GFP | 29.5 ± 5.0 (0.17) | 29.2 ± 4.7 (0.16) | 0.99 ± 0.05 (0.05) | 0.3383 |
| 0-ChFP/C00-GFP | 29.3 ± 3.7 (0.13) | 21.3 ± 3.0 (0.14) | 0.72 ± 0.04 (0.05) | 0.0004 |
| 0-ChFP/0(K52A)-GFP | 26.7 ± 4.8 (0.18) | 27.0 ± 5.3 (0.2) | 1.01 ± 0.07 (0.07) | 0.6349 |
| 0-ChFP/0(K85A)-GFP | 27.7 ± 2.8 (0.1) | 19.2 ± 2.9 (0.15) | 0.69 ± 0.06 (0.08) | 0.0001 |
| C-ChFP/C(K63A)-GFP | 24.4 ± 3.1 (0.13) | 14.6 ± 2.1 (0.07) | 0.60 ± 0.07 (0.08) | 0.0001 |
| C-ChFP/C(K96A)-GFP | 24.1 ± 6.5 (0.27) | 23.3 ± 6.7 (0.29) | 0.96 ± 0.06 (0.06) | 0.1316 |
| 00C-ChFP/00C-GFP | 27.0 ± 3.6 (0.13) | 27.1 ± 3.8 (0.12) | 1.01 ± 0.06 (0.06) | 0.7508 |
| 00C-ChFP/00(K52A)C-GFP | 28.4 ± 6.2 (0.22) | 14.9 ± 3.3 (0.22) | 0.53 ± 0.06 (0.11) | 0.0001 |
| 00C-ChFP/00(K85A)C-GFP | 30.2 ± 4.7 (0.15) | 29.5 ± 6.6 (0.22) | 0.96 ± 0.10 (0.10) | 0.3926 |
| CC0-ChFP/CC0-GFP | 22.3 ± 4.4 (0.19) | 22.1 ± 4.1 (0.19) | 1.00 ± 0.06 (0.06) | 0.7552 |
| CC0-ChFP/CC(K63A)0-GFP | 21.6 ± 2.9 (0.14) | 22.8 ± 2.5 (0.11) | 1.06 ± 0.05 (0.05) | 0.206 |
| CC0-ChFP/CC(K96A)0-GFP | 21.6 ± 3.7 (0.17) | 14.3 ± 1.7 (0.17) | 0.67 ± 0.06 (0.09) | 0.0001 |
| 0CC-ChFP/0CC-GFP | 25.2 ± 4.3 (0.17) | 26.1 ± 3.9 (0.15) | 1.04 ± 0.05 (0.05) | 0.6677 |
| 0CC-ChFP/0C(K63A)C-GFP | 25.0 ± 2.6 (0.14) | 13.7 ± 1.2 (0.09) | 0.55 ± 0.03 (0.06) | 0.0001 |
| 0CC-ChFP/0C(K96A)C-GFP | 25.7 ± 4.2 (0.16) | 26.8 ± 6.5 (0.24) | 1.04 ± 0.10 (0.10) | 0.2123 |
| C00-ChFP/C00-GFP | 22.9 ± 3.1 (0.14) | 23.6 ± 3.6 (0.15) | 1.03 ± 0.07 (0.07) | 0.6832 |
| C00-ChFP/C0(K85A)0-GFP | 23.0 ± 2.9 (0.13) | 15.6 ± 2.2 (0.14) | 0.69 ± 0.06 (0.10) | 0.0001 |
| C00-ChFP/C0(K52A)0-GFP | 24.3 ± 1.7 (0.07) | 16.0 ± 2.0 (0.13) | 0.66 ± 0.06 (0.09) | 0.0001 |
a The values for t50 were determined as previously described (35). The values are the means ± S.D. from at least eight independent measurements. The values in parentheses denote the calculated coefficient of variation.
b Paired Student's t test for H1-GFP versus H1-ChFP.